Enhancing the regenerative potential of stem cell-laden, clinical-grade implants through laminin engineering

Mogas Barcons, A; Chari, DM; Adams, CF

Abstract

Protected delivery of neural stem cells (NSCs; a major transplant population) within bioscaffolds has the potential to improve regenerative outcomes in sites of spinal cord injury. Emergent research has indicated clinical grade bioscaffolds (e.g. those used as surgical sealants) may be repurposed for this strategy, bypassing the long approval processes and difficulties in scale-up faced by laboratory grade materials. While promising, clinical scaffolds are often not inherently regenerative. Extracellular molecule biofunctionalisation of scaffolds can enhance regenerative features such as encapsulated cell survival/distribution, cell differentiation into desired cell types and nerve fibre growth. However, this strategy is yet to be tested for clinical grade scaffolds. Here, we show for the first time that Hemopatch™, a widely used, clinically approved surgical matrix, supports NSC growth. Further, functionalisation of Hemopatch™ with laminin promoted homogenous distribution of NSCs and their daughter cells within the matrix, a key regenerative criterion for transplant cells.

Citation

Mogas Barcons, A., Chari, D., & Adams, C. (2021). Enhancing the regenerative potential of stem cell-laden, clinical-grade implants through laminin engineering. Materials Science and Engineering: C, 123, https://doi.org/10.1016/j.msec.2021.111931

• Journal Article Type: Article
• Acceptance Date: Jan 28, 2021
• Publication Date: Apr 1, 2021
• Publicly Available Date: May 30, 2023
• Journal: Materials Science and Engineering: C
• Print ISSN: 0928-4931
• Electronic ISSN: 1873-0191
• Publisher: Elsevier
• Volume: 123
• DOI: https://doi.org/10.1016/j.msec.2021.111931
• Keywords: Extracellular matrix, Hemopatch™, Neural stem cell, Spinal cord injury, Surgical materials, Tissue engineering, Cell Differentiation, Humans, Laminin, Neural Stem Cells, Spinal Cord Injuries, Tissue Engineering, Tissue Scaffolds
• Publisher URL: https://www.sciencedirect.com/science/article/pii/S0928493121000692?via%3Dihub