Noninvasive imaging of nanoparticle-labeled transplant populations within polymer matrices for neural cell therapy

Tickle, Jacqueline A.; Poptani, Harish; Taylor, Arthur; Chari, Divya M.

Abstract

Aim: To develop a 3D neural cell construct for encapsulated delivery of transplant cells; develop hydrogels seeded with magnetic nanoparticle (MNP)-labeled cells suitable for cell tracking by MRI. Materials & methods: Astrocytes were exogenously labeled with MRI-compatible iron-oxide MNPs prior to intra-construct incorporation within a 3D collagen hydrogel. Results: A connective, complex cellular network was clearly observable within the 3D constructs, with high cellular viability. MNP accumulation in astrocytes provided a hypointense MRI signal at 24 h & 14 days. Conclusion: Our findings support the concept of developing a 3D construct possessing the dual advantages of (i) support of long-term cell survival of neural populations with (ii) the potential for noninvasive MRI-tracking of intra-construct cells for neuroregenerative applications.

Citation

Tickle, J. A., Poptani, H., Taylor, A., & Chari, D. M. (2018). Noninvasive imaging of nanoparticle-labeled transplant populations within polymer matrices for neural cell therapy. Nanomedicine, 13(11), 1333-1348. https://doi.org/10.2217/nnm-2017-0347

• Journal Article Type: Article
• Acceptance Date: Mar 29, 2018
• Online Publication Date: Jun 27, 2018
• Publication Date: 2018-06
• Deposit Date: Jun 23, 2023
• Journal: NANOMEDICINE
• Print ISSN: 1743-5889
• Publisher: Future Medicine
• Peer Reviewed: Peer Reviewed
• Volume: 13
• Issue: 11
• Pages: 1333-1348
• DOI: https://doi.org/10.2217/nnm-2017-0347