Harnessing a Biocatalyst to Bioremediate the Purification of Alkylglycosides.

Wahart, Alice J C; Dolan, Jonathan P; Anderson, Simon D; Cheallaigh, Aisling Ní; Staniland, Jessica; Lima, Marcelo A; Skidmore, Mark A; Miller, Gavin J; Cosgrove, Sebastian C

Abstract

As the world moves towards net-zero carbon emissions, the development of sustainable chemical manufacturing processes is essential. Within manufacturing, purification by distillation is often used, however this process is energy intensive and methods that could obviate or reduce its use are desirable. Developed herein is an alternative, oxidative biocatalytic approach that enables purification of alkyl monoglucosides (essential bio-based surfactant components). Implementing an immobilised engineered alcohol oxidase, a long-chain alcohol by-product derived from alkyl monoglucoside synthesis (normally removed by distillation) is selectively oxidised to an aldehyde, conjugated to an amine resin and then removed by simple filtration. This affords recovery of the purified alkyl monoglucoside. The approach lays a blueprint for further development of sustainable alkylglycoside purification using biocatalysis and, importantly, for refining other important chemical feedstocks that currently rely on distillation. [Abstract copyright: © 2023 The Authors. ChemBioChem published by Wiley-VCH GmbH.]

Citation

Wahart, A. J. C., Dolan, J. P., Anderson, S. D., Cheallaigh, A. N., Staniland, J., Lima, M. A., …Cosgrove, S. C. (in press). Harnessing a Biocatalyst to Bioremediate the Purification of Alkylglycosides. ChemBioChem, Article e202300625. https://doi.org/10.1002/cbic.202300625

• Journal Article Type: Article
• Acceptance Date: Oct 13, 2023
• Online Publication Date: Oct 13, 2023
• Deposit Date: Oct 31, 2023
• Journal: Chembiochem : a European journal of chemical biology
• Print ISSN: 1439-4227
• Electronic ISSN: 1439-7633
• Publisher: Wiley
• Peer Reviewed: Peer Reviewed
• Article Number: e202300625
• DOI: https://doi.org/10.1002/cbic.202300625
• Keywords: biocatalysis, sequestration, enzyme immobilisation, bioremediation, bioprocess optimisation