Oligodendrocyte progenitor cell (OPC) transplantation is unlikely to offer a means of preventing X-irradiation induced damage in the CNS

Chari, Divya M.; Gilson, Jennifer M.; Franklin, Robin J.M.; Blakemore, William F.

Abstract

Oligodendrocyte lineage cells [oligodendrocytes and their parent cells, the oligodendrocyte progenitor cells (OPCs)] are depleted by X-irradiation and progenitor cell transplantation has been proposed as a therapeutic strategy to counteract radiation induced myelopathy. Previous studies have demonstrated that oligodendrocyte progenitor cell (OPC) depletion is a prerequisite for establishing transplanted OPCs in normal tissue. One can therefore predict that the extent and timing of OPC depletion and regeneration following X-irradiation will be crucial factors in determining the feasibility of this therapeutic approach. To address this issue, we have examined the time course of OPC depletion and regeneration following a range of X-irradiation doses (5 to 40 Gy), and its relationship to establishing transplanted OPCs in X-irradiated tissue. Doses above 10 Gy resulted in rapid death of OPCs. With doses up to 20 Gy, surviving X-irradiated OPCs were capable of robust regeneration, restoring normal densities within 6 weeks. Transplanted OPCs could only be established in tissue that had been exposed to ≥20 Gy. Since 20 Gy is close to the ED50 for radiation necrosis, our findings demonstrate the limitation of OPC replacement strategies.

Citation

Chari, D. M., Gilson, J. M., Franklin, R. J., & Blakemore, W. F. (2006). Oligodendrocyte progenitor cell (OPC) transplantation is unlikely to offer a means of preventing X-irradiation induced damage in the CNS. Experimental Neurology, 198(1), 145-153. https://doi.org/10.1016/j.expneurol.2005.11.023

• Journal Article Type: Article
• Acceptance Date: Nov 22, 2005
• Online Publication Date: Jan 10, 2006
• Publication Date: 2006-03
• Deposit Date: Jun 11, 2024
• Journal: Experimental Neurology
• Print ISSN: 0014-4886
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 198
• Issue: 1
• Pages: 145-153
• DOI: https://doi.org/10.1016/j.expneurol.2005.11.023
• Public URL: https://keele-repository.worktribe.com/output/848911