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All Outputs (11)

In vitro and in vivo antimalarial activity and chemical profiling of sugarcane leaves. (2022)
Journal Article
Okokon, J. E., Mobley, R., Edem, U. A., Bassey, A. I., Fadayomi, I., Drijfhout, F., …Li, W. (2022). In vitro and in vivo antimalarial activity and chemical profiling of sugarcane leaves. Scientific reports, 10250 - ?. https://doi.org/10.1038/s41598-022-14391-8

Saccharum officinarum Linn. (sugarcane, Family-Poaceae) is employed in Ibibio traditional medicine for the treatment of various infections and diseases such as malaria. We This study aims to assess the antiplasmodial effect of the leaf extract and fr... Read More about In vitro and in vivo antimalarial activity and chemical profiling of sugarcane leaves..

Temperate zone plant natural products – a novel resource for activity against tropical parasitic diseases (2021)
Journal Article
Hameed, H., King, E., Doleckova, K., Bartholomew, B., Hollinshead, J., Mbye, H., …Price, H. (2021). Temperate zone plant natural products – a novel resource for activity against tropical parasitic diseases. Pharmaceuticals, 14(3), 1-17. https://doi.org/10.3390/ph14030227

The use of plant-derived natural products for the treatment of tropical parasitic diseases often has ethnopharmacological origins. As such, plants grown in temperate regions remain largely untested for novel anti-parasitic activities. We describe her... Read More about Temperate zone plant natural products – a novel resource for activity against tropical parasitic diseases.

Development of NanoLuc-PEST expressing Leishmania mexicana as a new drug discovery tool for axenic- and intramacrophage-based assays. (2018)
Journal Article
Price, H., Berry, S., Hameed, H., Thomason, A., Maciej-Hulme, M., Saif Abou-Akkada, S., & Horrocks, P. (2018). Development of NanoLuc-PEST expressing Leishmania mexicana as a new drug discovery tool for axenic- and intramacrophage-based assays. PLoS Neglected Tropical Diseases, 12(7), Article e0006639 . https://doi.org/10.1371/journal.pntd.0006639

The protozoan parasite Leishmania causes leishmaniasis; a spectrum of diseases of which there are an estimated 1 million new cases each year. Current treatments are toxic, expensive, difficult to administer, and resistance to them is emerging. New th... Read More about Development of NanoLuc-PEST expressing Leishmania mexicana as a new drug discovery tool for axenic- and intramacrophage-based assays..

G-quadruplex DNA motifs in the malaria parasite Plasmodium falciparum and their potential as novel antimalarial drug targets (2018)
Journal Article
Horrocks. (2018). G-quadruplex DNA motifs in the malaria parasite Plasmodium falciparum and their potential as novel antimalarial drug targets. Antimicrobial Agents and Chemotherapy, https://doi.org/10.1128/AAC.01828-17

G-quadruplexes are DNA or RNA secondary structures that can be formed from guanine-rich nucleic acids. These four-stranded structures, composed of stacked quartets of guanine bases, can be highly stable and have been demonstrated to occur in vivo in... Read More about G-quadruplex DNA motifs in the malaria parasite Plasmodium falciparum and their potential as novel antimalarial drug targets.

Identification of heparin modifications and polysaccharide inhibitors of Plasmodium falciparum merozoite invasion that have potential for novel drug development. (2017)
Journal Article
Boyle, M. J., Skidmore, M., Dickerman, B., Cooper, L., Devlin, A., Yates, E., …Beeson, J. G. (2017). Identification of heparin modifications and polysaccharide inhibitors of Plasmodium falciparum merozoite invasion that have potential for novel drug development. Antimicrobial Agents and Chemotherapy, 61(11), https://doi.org/10.1128/AAC.00709-17

Despite recent successful control efforts, malaria remains a leading global health burden. Alarmingly, resistance to current antimalarials is increasing, and the development of new drug families is needed to maintain malaria control. Current antimala... Read More about Identification of heparin modifications and polysaccharide inhibitors of Plasmodium falciparum merozoite invasion that have potential for novel drug development..

Triaging informative cis -regulatory elements for the combinatorial control of temporal gene expression during Plasmodium falciparum intraerythrocytic development (2015)
Journal Article
Horrocks. (2015). Triaging informative cis -regulatory elements for the combinatorial control of temporal gene expression during Plasmodium falciparum intraerythrocytic development. Parasites and Vectors, 81 -?. https://doi.org/10.1186/s13071-015-0701-0

BackgroundOver 2700 genes are subject to stage-specific regulation during the intraerythrocytic development of the human malaria parasite Plasmodium falciparum. Bioinformatic analyses have identified a large number of over-represented motifs in the 5... Read More about Triaging informative cis -regulatory elements for the combinatorial control of temporal gene expression during Plasmodium falciparum intraerythrocytic development.

Homopolymer tract organization in the human malarial parasite Plasmodium falciparum and related Apicomplexan parasites (2014)
Journal Article
Horrocks. (2014). Homopolymer tract organization in the human malarial parasite Plasmodium falciparum and related Apicomplexan parasites. BMC Genomics, 848 -?. https://doi.org/10.1186/1471-2164-15-848

BACKGROUND: Homopolymeric tracts, particularly poly dA.dT, are enriched within the intergenic sequences of eukaryotic genomes where they appear to act as intrinsic regulators of nucleosome positioning. A previous study of the incomplete genome of the... Read More about Homopolymer tract organization in the human malarial parasite Plasmodium falciparum and related Apicomplexan parasites.

Evaluation of bioluminescence-based assays of anti-malarial drug activity (2013)
Journal Article
Horrocks. (2013). Evaluation of bioluminescence-based assays of anti-malarial drug activity. Malaria Journal, 58 - ?. https://doi.org/10.1186/1475-2875-12-58

Transgenic Plasmodium falciparum expressing luciferase offers an attractive bioluminescence-based assay platform for the investigation of the pharmacological properties of anti-malarial drugs. Here a side-by-side comparison of bioluminescence and flu... Read More about Evaluation of bioluminescence-based assays of anti-malarial drug activity.

Comparison of the absolute and relative efficiencies of electroporation-based transfection protocols for Plasmodium falciparum. (2012)
Journal Article
Horrocks. (2012). Comparison of the absolute and relative efficiencies of electroporation-based transfection protocols for Plasmodium falciparum. Malaria Journal, 210 - ?. https://doi.org/10.1186/1475-2875-11-210

BACKGROUND: Several electroporation protocols exist to transfect exogenous DNA into Plasmodium falciparum. To date, however, only a subjective analysis of their relative efficiencies has been reported. METHODS: A time-course of luciferase reporter ex... Read More about Comparison of the absolute and relative efficiencies of electroporation-based transfection protocols for Plasmodium falciparum..

Deciphering the ubiquitin-mediated pathway in apicomplexan parasites: a potential strategy to interfere with parasite virulence (2008)
Journal Article
Horrocks. (2008). Deciphering the ubiquitin-mediated pathway in apicomplexan parasites: a potential strategy to interfere with parasite virulence. PloS one, e2386 - ?. https://doi.org/10.1371/journal.pone.0002386

Reversible modification of proteins through the attachment of ubiquitin or ubiquitin-like modifiers is an essential post-translational regulatory mechanism in eukaryotes. The conjugation of ubiquitin or ubiquitin-like proteins has been demonstrated t... Read More about Deciphering the ubiquitin-mediated pathway in apicomplexan parasites: a potential strategy to interfere with parasite virulence.