Telomerase inhibition, telomere attrition and proliferation arrest of cancer cells induced by phosphorothioate ASO-NLS conjugates targeting hTERC and siRNAs targeting hTERT

Diala, Irmina; Shiohama, Yasuo; Fujita, Takashi; Kotake, Yojiro; Demonacos, Constantinos; Krstic-Demonacos, Marija; Di Leva, Gianpiero; Fujii, Masayuki

Abstract

Telomerase activity has been regarded as a critical step in cellular immortalization and carcinogenesis and because of this, regulation of telomerase represents an attractive target for anti-tumor specific therapeutics. Recently, one avenue of cancer research focuses on antisense strategy to target the oncogenes or cancer driver genes, in a sequence specific fashion to down-regulate the expression of the target gene. The protein catalytic subunit, human telomerase reverse transcriptase (hTERT) and the template RNA component (hTERC) are essential for telomerase function, thus theoretically, inhibition of telomerase activity can be achieved by interfering with either the gene expression of hTERT or the hTERC of the telomerase enzymatic complex. The present study showed that phosphorothioate antisense oligonucleotide (sASO)-nuclear localization signal (NLS) peptide conjugates targeting hTERC could inhibit telomerase activity very efficiently at 5?µM concentration but less efficiently at 1?µM concentration. On the other hand, siRNA targeting hTERT mRNA could strongly suppress hTERT expression at 200?nM concentration. It was also revealed that siRNA targeting hTERT could induce telomere attrition and then irreversible arrest of proliferation of cancer cells.

Citation

Diala, I., Shiohama, Y., Fujita, T., Kotake, Y., Demonacos, C., Krstic-Demonacos, M., …Fujii, M. (2020). Telomerase inhibition, telomere attrition and proliferation arrest of cancer cells induced by phosphorothioate ASO-NLS conjugates targeting hTERC and siRNAs targeting hTERT. Nucleosides, Nucleotides and Nucleic Acids, 39(1-3), 407 - 425. https://doi.org/10.1080/15257770.2020.1713357

• Journal Article Type: Article
• Acceptance Date: Jan 5, 2020
• Publication Date: Apr 20, 2020
• Journal: Nucleosides, Nucleotides & Nucleic Acids
• Print ISSN: 1525-7770
• Publisher: Taylor and Francis
• Peer Reviewed: Peer Reviewed
• Volume: 39
• Issue: 1-3
• Pages: 407 - 425
• DOI: https://doi.org/10.1080/15257770.2020.1713357
• Keywords: Cancer cells, telomerase/telomeres, proliferation arrest, hTERT, hTERC, sASO-NLS conjugate, siRNA
• Publisher URL: https://www.tandfonline.com/doi/full/10.1080/15257770.2020.1713357