681 USING NANOPORE SEQUENCING TO DIAGNOSE PROSTHETIC JOINT INFECTIONS
(2024)
Presentation / Conference
Outputs (58)
437 CHARACTERISATION OF JUVENILE CARTILAGE TISSUES AND THEIR ISOLATED CHONDROCYTES FOR ALLOGENEIC CHONDROCYTE THERAPY (2024)
Presentation / Conference
Using Nanopore sequencing to identify bacterial infection in joint replacements: A Preliminary study (2024)
Journal Article
This project investigates if third-generation genomic sequencing can be used to identify the species of bacteria causing prosthetic joint infections (PJIs) at the time of revision surgery. Samples of prosthetic fluid were taken during revision surger... Read More about Using Nanopore sequencing to identify bacterial infection in joint replacements: A Preliminary study.
CHARACTERIZING CELLULAR COMPONENTS OF BONE MARROW ASPIRATE CONCENTRATE: A ONE-STEP CELL THERAPY FOR ANKLE CARTILAGE DEFECTS (2023)
Journal Article
AbstractObjectivesBone marrow aspirate concentrate (BMAC), together with fibrin glue (Tisseel, Baxter, UK) and Hyaluronic acid (HA) were used as a one-step cell therapy treating patients with ankle cartilage defects in our hospital. This therapy was... Read More about CHARACTERIZING CELLULAR COMPONENTS OF BONE MARROW ASPIRATE CONCENTRATE: A ONE-STEP CELL THERAPY FOR ANKLE CARTILAGE DEFECTS.
SYNOVIAL FLUID PROTEOMICS AND BIOINFORMATICS HIGHLIGHTS POTENTIAL MECHANISMS OF UMBILICAL CORD-MSC INDUCED RADIOLOGICAL IMPROVEMENT IN AN OVINE MODEL OF OSTEOARTHRITIS (2023)
Journal Article
AbstractObjectivesA promising therapy for early osteoarthritis (OA) is the transplantation of human umbilical cord-derived mesenchymal stromal cells (hUC-MSCs). The synovial fluid (SF) from a pre-clinical ovine model treated with hUC-MSCs has been pr... Read More about SYNOVIAL FLUID PROTEOMICS AND BIOINFORMATICS HIGHLIGHTS POTENTIAL MECHANISMS OF UMBILICAL CORD-MSC INDUCED RADIOLOGICAL IMPROVEMENT IN AN OVINE MODEL OF OSTEOARTHRITIS.
Analysis of Synovial Fluid Proteomes After Treating an Ovine Osteoarthritic Model with Human Umbilical Cord Mesenchymal Stem Cells (2023)
Presentation / Conference
The Up-Scale Manufacture of Chondrocytes for Allogeneic Cartilage Therapies (2023)
Journal Article
Background: Allogeneic chondrocyte therapies need to be developed to allow more individuals to be treated with a cell therapy for cartilage repair and to reduce the burden and cost of current two-stage autologous procedures. Up-scale manufacture of c... Read More about The Up-Scale Manufacture of Chondrocytes for Allogeneic Cartilage Therapies.
Proteomic Profiling of Different Clinical Responses to Surgically Sustained and Acute Traumatic Cartilage Injuries (2023)
Conference Proceeding
A comprehensive review of quantum bioreactor cell manufacture: Research and clinical applications (2023)
Journal Article
The Quantum cell expansion system manufactured by Terumo-BCT is perhaps the most widely reported Good Manufacturing Practice–compliant bioreactor used for the expansion of adherent cell populations, both for research purposes and cl... Read More about A comprehensive review of quantum bioreactor cell manufacture: Research and clinical applications.
PROTEOMIC IDENTIFICATION OF PREDICTIVE AUTOLOGOUS CHONDROCYTE IMPLANTATION BIOMARKERS IN PLASMA (2023)
Journal Article
Stratification is required to ensure that only those patients likely to benefit, receive Autologous Chondrocyte Implantation (ACI); ideally by assessing a biomarker in the blood. This study aimed to assess differences in the plasma proteome of indivi... Read More about PROTEOMIC IDENTIFICATION OF PREDICTIVE AUTOLOGOUS CHONDROCYTE IMPLANTATION BIOMARKERS IN PLASMA.
Proteomic Analyses of Autologous Chondrocyte Implantation Plasma Highlight Cartilage Acidic Protein 1 as a Candidate for Preclinical Screening. (2023)
Journal Article
BACKGROUND: Stratification is required to ensure that only patients likely to benefit receive autologous chondrocyte implantation (ACI). It would be advantageous to identify biomarkers to predict ACI outcome that are measurable in blood, avoiding the... Read More about Proteomic Analyses of Autologous Chondrocyte Implantation Plasma Highlight Cartilage Acidic Protein 1 as a Candidate for Preclinical Screening..
Chondrocyte Isolation and Expansion (2022)
Book Chapter
Chondrocyte isolation requires a combination of enzymatic and mechanical digestion of cartilaginous tissues in order to release the chondrocytes. Extracted primary chondrocytes will then adhere to standard tissue culture plastics, typically in small... Read More about Chondrocyte Isolation and Expansion.
1Abstract1.1IntroductionSpinal Cord Injury (SCI) is a major cause of disability, with complications post-injury often leading to life-long health issues with need of extensive treatment. Neurological outcome post-SCI can be variable and difficult to... Read More about A comprehensive proteomic and bioinformatics analysis of human spinal cord injury plasma identifies proteins associated with the complement cascade and liver function as potential prognostic indicators of neurological outcome.
Assessing allogeneic chondroprogenitor manufacture in xeno-free and serum-free media (2022)
Presentation / Conference
Investigation of the blood proteome in response to spinal cord injury in rodent models (2021)
Journal Article
STUDY DESIGN: Explanatory and Mechanistic Study OBJECTIVES: A better understanding of the ‘whole-body’ response following spinal cord injury (SCI) is needed to guide future research aimed at developing novel therapeutic interventions and identifying... Read More about Investigation of the blood proteome in response to spinal cord injury in rodent models.
Identification of Candidate Synovial Fluid Biomarkers for the Prediction of Patient Outcome following Microfracture or Osteotomy (2021)
Journal Article
Background: Biomarkers are needed to predict clinical outcome for microfracture and osteotomy surgeries to ensure patients can be better stratified to receive the most appropriate treatment. Hypothesis/purpose: To identify novel biomarker candidate... Read More about Identification of Candidate Synovial Fluid Biomarkers for the Prediction of Patient Outcome following Microfracture or Osteotomy.
The Management of Talar Osteochondral Lesions - Current Concepts (2021)
Journal Article
Osteochondral lesions of the talus (OLTs) are a common complication following trauma, involving both the articular cartilage and the underlying subchondral bone, with variable aetiologies and often presenting with non-specific symptoms. Diagnosis of... Read More about The Management of Talar Osteochondral Lesions - Current Concepts.
Identification of Candidate Synovial Fluid Biomarkers for the Prediction of Patient Outcome After Microfracture or Osteotomy (2021)
Journal Article
Background: Biomarkers are needed to predict clinical outcomes for microfracture and osteotomy surgeries to ensure patients can be better stratified to receive the most appropriate treatment. Purpose: To identify novel biomarker candidates and to... Read More about Identification of Candidate Synovial Fluid Biomarkers for the Prediction of Patient Outcome After Microfracture or Osteotomy.
Synovial Fluid Biomarkers for the Prediction of Patient Outcome following Microfracture or Osteotomy (2021)
Journal Article
Objectives The ability to predict which patients will improve following routine surgeries aimed at preventing the progression of osteoarthritis is needed to aid patients being stratified to receive the most appropriate treatment. This study aimed to... Read More about Synovial Fluid Biomarkers for the Prediction of Patient Outcome following Microfracture or Osteotomy.
Evaluating patient perspectives on participating in scientific research and clinical trials for the treatment of spinal cord injury (2021)
Journal Article
A questionnaire was developed to evaluate patients' perspective on research aimed at improving functions and overcoming complications associated with spinal cord injury (SCI). The first three sections were based on published and validated assessment... Read More about Evaluating patient perspectives on participating in scientific research and clinical trials for the treatment of spinal cord injury.
Routinely Measured Hematological Markers Can Help to Predict American Spinal Injury Association Impairment Scale Scores after Spinal Cord Injury (2020)
Journal Article
Neurological outcomes following spinal cord injury (SCI) are currently difficult to predict. Whilst the initial American Spinal Injury Association (ASIA) Impairment Scale (AIS) grade can give an estimate of outcome, the high remaining degree of uncer... Read More about Routinely Measured Hematological Markers Can Help to Predict American Spinal Injury Association Impairment Scale Scores after Spinal Cord Injury.
The synovial fluid from patients with focal cartilage defects contains mesenchymal stem/stromal cells and macrophages with pro- and anti-inflammatory phenotypes (2020)
Journal Article
Objective The synovial fluid (SF) of patients with focal cartilage defects contains a population of poorly characterised cells that could have pathophysiological implications in early osteoarthritis and joint tissue repair. We have examined the cells... Read More about The synovial fluid from patients with focal cartilage defects contains mesenchymal stem/stromal cells and macrophages with pro- and anti-inflammatory phenotypes.
The effect of high glucose on lipid metabolism in the human placenta (2019)
Journal Article
Diabetes mellitus (DM) during pregnancy can result in fetal overgrowth, likely due to placental dysfunction, which has health consequences for the infant. Here we test our prediction from previous work using a placental cell line that high glucose co... Read More about The effect of high glucose on lipid metabolism in the human placenta.
A Preliminary Cohort Study Assessing Routine Blood Analyte Levels and Neurological Outcome after Spinal Cord Injury. (2019)
Journal Article
There is increasing interest in the identification of biomarkers that could predict neurological outcome following a spinal cord injury (SCI). Although initial American Spinal Injury Association (ASIA) Impairment Scale (AIS) grade is a good indicator... Read More about A Preliminary Cohort Study Assessing Routine Blood Analyte Levels and Neurological Outcome after Spinal Cord Injury..
Combined Autologous Chondrocyte and Bone Marrow Mesenchymal Stromal Cell Implantation in the Knee: An 8-year Follow Up of Two First-In-Man Cases (2019)
Journal Article
Autologous chondrocyte implantation (ACI) has been used to treat cartilage defects for >20 years, with promising clinical outcomes. Here, we report two first-in-man cases (patient A and B) treated with combined autologous chondrocyte and bone marrow... Read More about Combined Autologous Chondrocyte and Bone Marrow Mesenchymal Stromal Cell Implantation in the Knee: An 8-year Follow Up of Two First-In-Man Cases.
A comprehensive characterisation of large-scale expanded human bone marrow and umbilical cord mesenchymal stem cells. (2019)
Journal Article
BACKGROUND: The manufacture of mesenchymal stem/stromal cells (MSCs) for clinical use needs to be cost effective, safe and scaled up. Current methods of expansion on tissue culture plastic are labour-intensive and involve several 'open' procedures.... Read More about A comprehensive characterisation of large-scale expanded human bone marrow and umbilical cord mesenchymal stem cells..
Up-scale manufacture of chondrocytes in the development of allogeneic cartilage therapies (2018)
Presentation / Conference
Identification of synovial fluid proteins that are associated with early osteoarthritis treatment failure: the search for novel markers leads us back to matrix metalloproteinases (MMPs) (2018)
Presentation / Conference
Introduction: Autologous chondrocyte implantation (ACI) is a cell therapy used to treat cartilage defects and early osteoarthritis. During initial surgery (Stage I), healthy cartilage is harvested from the joint. Chondrocytes are isolated and culture... Read More about Identification of synovial fluid proteins that are associated with early osteoarthritis treatment failure: the search for novel markers leads us back to matrix metalloproteinases (MMPs).
Two independent proteomic approaches provide a comprehensive analysis of the synovial fluid proteome response to Autologous Chondrocyte Implantation (2018)
Journal Article
Background Autologous chondrocyte implantation (ACI) has a failure rate of approximately 20%, but it is yet to be fully understood why. Biomarkers are needed that can pre-operatively predict in which patients it is likely to fail, so that alternativ... Read More about Two independent proteomic approaches provide a comprehensive analysis of the synovial fluid proteome response to Autologous Chondrocyte Implantation.
Acute phase response signalling is altered following cartilage harvest in non-responders to Autologous Chondrocyte Implantation (2018)
Journal Article
Background: Autologous chondrocyte implantation (ACI) has a failure rate of approximately 20%, but it is yet to be fully understood why. Biomarkers are needed that can pre-operatively predict in which patients it is likely to fail, so that alternat... Read More about Acute phase response signalling is altered following cartilage harvest in non-responders to Autologous Chondrocyte Implantation.
Assessment of candidate predictive protein biomarkers for microfracture and osteotomy (2018)
Journal Article
PlumX Metrics Purpose: Biomarkers that can predict clinical outcome for surgeries to prevent the progression of osteoarthritis (OA) are needed to ensure patients can be better stratified to receive the most appropriate treatment. We have previousl... Read More about Assessment of candidate predictive protein biomarkers for microfracture and osteotomy.
Identification of the functional pathways altered by placental cell exposure to high glucose: lessons from the transcript and metabolite interactome (2018)
Journal Article
The specific consequences of hyperglycaemia on placental metabolism and function are incompletely understood but likely contribute to poor pregnancy outcomes associated with diabetes mellitus (DM). This study aimed to identify the functional biochemi... Read More about Identification of the functional pathways altered by placental cell exposure to high glucose: lessons from the transcript and metabolite interactome.
Similar phenotypes of cells in the repair tissues following microfracture or autologous chondrocyte implantation (2017)
Journal Article
Introduction Autologous chondrocyte implantation (ACI) and microfracture are both used in the treatment of cartilage injuries. Previous findings suggest that the efficacy of these two therapies is similar in terms of short-term clinical outcome, h... Read More about Similar phenotypes of cells in the repair tissues following microfracture or autologous chondrocyte implantation.
Autologous chondrocyte implantation- derived synovial fluids display distinct responder and non-responder proteomic profiles (2017)
Journal Article
Background Autologous chondrocyte implantation (ACI) can be used in the treatment of focal cartilage injuries to prevent the onset of osteoarthritis (OA). However, we are yet to understand fully why some individuals do not respond well to this interv... Read More about Autologous chondrocyte implantation- derived synovial fluids display distinct responder and non-responder proteomic profiles.
Cartilage harvest elevates sCD14 and MMP-3 in the synovial fluid of the autologous chondrocyte implantation (ACI) treated joint (2017)
Journal Article
Introduction This study aims to examine the impact of the cartilage harvest procedure on synovial fluid biomarkers at the time of autologous chondrocyte implantation (ACI). Materials and Methods Hyaluronic acid (HA), cartilage oligomeric matrix pr... Read More about Cartilage harvest elevates sCD14 and MMP-3 in the synovial fluid of the autologous chondrocyte implantation (ACI) treated joint.
The Developing Landscape of Diagnostic and Prognostic Biomarkers for Spinal Cord Injury in Cerebrospinal Fluid and Blood (2016)
Journal Article
Objectives: The identification of prognostic biomarkers of spinal cord injury (SCI) will help to assign SCI patients to the correct treatment and rehabilitation regimes. Further, the detection of biomarkers that predict permanent neurological outcom... Read More about The Developing Landscape of Diagnostic and Prognostic Biomarkers for Spinal Cord Injury in Cerebrospinal Fluid and Blood.
Cell Therapy for Cartilage Repair
Journal Article
Regenerative medicine, using cells as therapeutic agents for the repair or regeneration of tissues and organs, offers great hope for the future of medicine. Cell therapy for treating defects in articular cartilage has been an exemplar of translating... Read More about Cell Therapy for Cartilage Repair.
Juvenile Chondrocytes: Novel Alternatives for Allogeneic Cell Therapy?
Presentation / Conference
Purpose Development of novel allogeneic chondrocyte therapies are needed to provide a more widespread, cost-effective cartilage treatment option. Here we investigate the potential of juvenile cartilage sources for allogeneic chondrocyte manufactur... Read More about Juvenile Chondrocytes: Novel Alternatives for Allogeneic Cell Therapy?.
The Up-Scale Manufacture of Chondrocytes for Allogeneic Cartilage Therapies
Presentation / Conference
INTRODUCTION: Up-scaled allogeneic cellular products for cartilage therapies, along with identification of novel young donors of healthy chondrocytes, are needed to improve the cost effectiveness and wide-spread use of chondrocyte therapy. METHODS... Read More about The Up-Scale Manufacture of Chondrocytes for Allogeneic Cartilage Therapies.
IS ORTHOPAEDICS LEADING THE WAY IN GETTING CELL THERAPY TO THE CLINIC?
Presentation / Conference