Outputs (39)
SYNOVIAL FLUID PROTEOMICS AND BIOINFORMATICS HIGHLIGHTS POTENTIAL MECHANISMS OF UMBILICAL CORD-MSC INDUCED RADIOLOGICAL IMPROVEMENT IN AN OVINE MODEL OF OSTEOARTHRITIS (2023)
Journal Article
AbstractObjectivesA promising therapy for early osteoarthritis (OA) is the transplantation of human umbilical cord-derived mesenchymal stromal cells (hUC-MSCs). The synovial fluid (SF) from a pre-clinical ovine model treated with hUC-MSCs has been pr... Read More about SYNOVIAL FLUID PROTEOMICS AND BIOINFORMATICS HIGHLIGHTS POTENTIAL MECHANISMS OF UMBILICAL CORD-MSC INDUCED RADIOLOGICAL IMPROVEMENT IN AN OVINE MODEL OF OSTEOARTHRITIS.
Analysis of Synovial Fluid Proteomes After Treating an Ovine Osteoarthritic Model with Human Umbilical Cord Mesenchymal Stem Cells (2023)
Presentation / Conference
Extracellular vesicles derived from umbilical cord mesenchymal stromal cells show enhanced anti-inflammatory properties via upregulation of miRNAs after pro-inflammatory priming (2023)
Journal Article
Autoimmune conditions, such as rheumatoid arthritis, are characterised by a loss of immune tolerance, whereby the immune cells attack self-antigens causing pain and inflammation. These conditions can be brought into remission using pharmaceutical tre... Read More about Extracellular vesicles derived from umbilical cord mesenchymal stromal cells show enhanced anti-inflammatory properties via upregulation of miRNAs after pro-inflammatory priming.
The Up-Scale Manufacture of Chondrocytes for Allogeneic Cartilage Therapies (2023)
Journal Article
Background: Allogeneic chondrocyte therapies need to be developed to allow more individuals to be treated with a cell therapy for cartilage repair and to reduce the burden and cost of current two-stage autologous procedures. Up-scale manufacture of c... Read More about The Up-Scale Manufacture of Chondrocytes for Allogeneic Cartilage Therapies.
Extracellular Vesicle Depletion Protocols of Foetal Bovine Serum Influence Umbilical Cord Mesenchymal Stromal Cell Phenotype, Immunomodulation, and Particle Release (2023)
Journal Article
The immunomodulatory properties of MSCs can be recreated using their extracellular vesicles (EVs). Yet, the true capabilities of the MSC EVs cannot be distinguished from contaminating bovine EVs and protein derived from supplemental foetal bovine ser... Read More about Extracellular Vesicle Depletion Protocols of Foetal Bovine Serum Influence Umbilical Cord Mesenchymal Stromal Cell Phenotype, Immunomodulation, and Particle Release.
A comprehensive review of quantum bioreactor cell manufacture: Research and clinical applications (2023)
Journal Article
The Quantum cell expansion system manufactured by Terumo-BCT is perhaps the most widely reported Good Manufacturing Practice–compliant bioreactor used for the expansion of adherent cell populations, both for research purposes and cl... Read More about A comprehensive review of quantum bioreactor cell manufacture: Research and clinical applications.
Human Mesenchymal Stromal Cells Enhance Cartilage Healing in a Murine Joint Surface Injury Model. (2021)
Journal Article
Human umbilical cord (hUC)- or bone marrow (hBM)-derived mesenchymal stromal cells (MSCs) were evaluated as an allogeneic source of cells for cartilage repair. We aimed to determine if they could enhance healing of chondral defects with or without th... Read More about Human Mesenchymal Stromal Cells Enhance Cartilage Healing in a Murine Joint Surface Injury Model..
The Use of Technology in the Subcategorisation of Osteoarthritis: a Delphi Study Approach (2020)
Journal Article
Objective This UK-wide OATech+ Network consensus study utilised a Delphi approach to discern levels of awareness across an expert panel regarding the role of existing and novel technologies in osteoarthritis research. To direct future cross-disciplin... Read More about The Use of Technology in the Subcategorisation of Osteoarthritis: a Delphi Study Approach.
The synovial fluid from patients with focal cartilage defects contains mesenchymal stem/stromal cells and macrophages with pro- and anti-inflammatory phenotypes (2020)
Journal Article
Objective The synovial fluid (SF) of patients with focal cartilage defects contains a population of poorly characterised cells that could have pathophysiological implications in early osteoarthritis and joint tissue repair. We have examined the cells... Read More about The synovial fluid from patients with focal cartilage defects contains mesenchymal stem/stromal cells and macrophages with pro- and anti-inflammatory phenotypes.
Pro-Inflammatory Priming of Umbilical Cord Mesenchymal Stromal Cells Alters the Protein Cargo of Their Extracellular Vesicles (2020)
Journal Article
Umbilical cord mesenchymal stromal cells (UCMSCs) have shown an ability to modulate the immune system through the secretion of paracrine mediators, such as extracellular vesicles (EVs). However, the culture conditions that UCMSCs are grown in can alt... Read More about Pro-Inflammatory Priming of Umbilical Cord Mesenchymal Stromal Cells Alters the Protein Cargo of Their Extracellular Vesicles.
Injected human umbilical cord-derived mesenchymal stromal cells do not appear to elicit an inflammatory response in a murine model of osteoarthritis (2020)
Journal Article
Objective This study investigated the effect of hUC-MSCs on osteoarthritis (OA) progression in a xenogeneic model. Design Male, 10 week-old C57Bl/6 mice underwent sham surgery (n=15) or partial medial meniscectomy (PMM;n=76). 5x105 hUC-MSCs (from 3 d... Read More about Injected human umbilical cord-derived mesenchymal stromal cells do not appear to elicit an inflammatory response in a murine model of osteoarthritis.
Human Articular Chondrocytes Retain Their Phenotype in Sustained Hypoxia While Normoxia Promotes Their Immunomodulatory Potential (2019)
Journal Article
Objective To assess the phenotype of human articular chondrocytes cultured in normoxia (21% O2) or continuous hypoxia (2% O2). Design Chondrocytes were extracted from patients undergoing total knee replacement (n = 5) and cultured in ~21% (normo... Read More about Human Articular Chondrocytes Retain Their Phenotype in Sustained Hypoxia While Normoxia Promotes Their Immunomodulatory Potential.
A comprehensive characterisation of large-scale expanded human bone marrow and umbilical cord mesenchymal stem cells. (2019)
Journal Article
BACKGROUND: The manufacture of mesenchymal stem/stromal cells (MSCs) for clinical use needs to be cost effective, safe and scaled up. Current methods of expansion on tissue culture plastic are labour-intensive and involve several 'open' procedures.... Read More about A comprehensive characterisation of large-scale expanded human bone marrow and umbilical cord mesenchymal stem cells..
Up-scale manufacture of chondrocytes in the development of allogeneic cartilage therapies (2018)
Presentation / Conference
Magnetic Resonance Imaging Parameters at 1 Year Correlate With Clinical Outcomes Up to 17 Years After Autologous Chondrocyte Implantation (2018)
Journal Article
Background: The ability to predict the long-term success of surgical treatment in orthopaedics is invaluable, particularly in clinical trials. The quality of repair tissue formed 1 year after autologous chondrocyte implantation (ACI) in the knee was... Read More about Magnetic Resonance Imaging Parameters at 1 Year Correlate With Clinical Outcomes Up to 17 Years After Autologous Chondrocyte Implantation.
Characterisation of synovial fluid cells of early osteoarthritis patients (2018)
Journal Article
Characterisation of joint macrophages: implications for osteoarthritis (2017)
Journal Article
Introduction Inflammation in the synovium is a feature of osteoarthritis (OA) that contributes to the general degradation of the joint by the secretion of pro-inflammatory molecules and degradative enzymes. The infrapatellar fat pad (IPFP) is an intr... Read More about Characterisation of joint macrophages: implications for osteoarthritis.
Human articular chondrocytes retain their phenotype in sustained hypoxia whilst normoxia promotes their immunomodulatory capacity (2017)
Presentation / Conference
Introduction The maintenance of chondrogenic phenotype in human articular chondrocytes grown in continuous normoxic (21% O2) and hypoxic (2% O2) conditions was assessed, with the aim of finding the best environment for culturing cells destined for ca... Read More about Human articular chondrocytes retain their phenotype in sustained hypoxia whilst normoxia promotes their immunomodulatory capacity.
Ageing in the musculoskeletal system (2016)
Journal Article
The extent of ageing in the musculoskeletal system during the life course affects the quality and length of life. Loss of bone, degraded articular cartilage, and degenerate, narrowed intervertebral discs are primary features of an ageing skeleton, an... Read More about Ageing in the musculoskeletal system.
Chondrogenic Potency Analyses of Donor-Matched Chondrocytes and Mesenchymal Stem Cells Derived from Bone Marrow, Infrapatellar Fat Pad, and Subcutaneous Fat. (2016)
Journal Article
Autologous chondrocyte implantation (ACI) is a cell-based therapy that has been used clinically for over 20 years to treat cartilage injuries more efficiently in order to negate or delay the need for joint replacement surgery. In this time, very litt... Read More about Chondrogenic Potency Analyses of Donor-Matched Chondrocytes and Mesenchymal Stem Cells Derived from Bone Marrow, Infrapatellar Fat Pad, and Subcutaneous Fat..
Mesenchymal stromal cells derived from whole human umbilical cord exhibit similar properties to those derived from Wharton’s jelly and bone marrow (2016)
Journal Article
Mesenchymal stromal cells (MSC) can be isolated from several regions of human umbilical cords, including Wharton's jelly (WJ), artery, vein or cord lining. These MSC appear to be immune privileged and are promising candidates for cell therapy. Howeve... Read More about Mesenchymal stromal cells derived from whole human umbilical cord exhibit similar properties to those derived from Wharton’s jelly and bone marrow.
Mesenchymal stromal cells from human umbilical cords display poor chondrogenic potential in scaffold-free three dimensional cultures. (2016)
Journal Article
Many researchers world over are currently investigating the suitability of stromal cells harvested from foetal tissues for allogeneic cell transplantation therapies or for tissue engineering purposes. In this study, we have investigated the chondroge... Read More about Mesenchymal stromal cells from human umbilical cords display poor chondrogenic potential in scaffold-free three dimensional cultures..
Characterisation of synovial fluid and infrapatellar fat pad derived mesenchymal stromal cells: The influence of tissue source and inflammatory stimulus. (2016)
Journal Article
The infrapatellar fat pad (FP) and synovial fluid (SF) in the knee serve as reservoirs of mesenchymal stromal cells (MSCs) with potential therapeutic benefit. We determined the influence of the donor on the phenotype of donor matched FP and SF derive... Read More about Characterisation of synovial fluid and infrapatellar fat pad derived mesenchymal stromal cells: The influence of tissue source and inflammatory stimulus..
Obesity does not affect the size of infrapatellar fat pad adipocytes: implications for the pathogenesis of knee osteoarthritis (2016)
Journal Article
Purpose: Obesity is known to be a risk factor for the development of osteoarthritis (OA). Obesity is accompanied by adipocyte hypertrophy and elevated inflammation in subcutaneous and visceral adipose tissue. The infrapatellar fat pad (IPFP) is an ad... Read More about Obesity does not affect the size of infrapatellar fat pad adipocytes: implications for the pathogenesis of knee osteoarthritis.
Human umbilical cord stem cells: preliminary characterisation in an inflammatory environment (2014)
Conference Proceeding
Cells isolated from fat pad and synovial fluid. are they suitable for cartilage repair? (2014)
Journal Article
Purpose: Autologous Chondrocyte Implantation (ACI) is used as a cellular therapy for treating defects in articular cartilage. Successful ACI depends on high cell number and quality of the cells which varies between patients. Alternative cell sources... Read More about Cells isolated from fat pad and synovial fluid. are they suitable for cartilage repair?.
Are umbilical cord mesenchymal stem cells advantageous over bone marrow mesenchymal stem cells for cell therapy in orthopaedics? (2014)
Conference Proceeding
Purpose: Mesenchymal Stem cells (MSC) from umbilical cords (UC) are of increasing interest for cell therapy in degenerative musculoskeletal disorders as they present little ethical consideration and are reported to contain immune privileged cells, wh... Read More about Are umbilical cord mesenchymal stem cells advantageous over bone marrow mesenchymal stem cells for cell therapy in orthopaedics?.
Characterization of the cells in repair tissue following autologous chondrocyte implantation in mankind: a novel report of two cases (2013)
Journal Article
AIM: Autologous chondrocyte implantation (ACI) is used worldwide for the treatment of cartilage defects. This study has aimed to assess for the first time the cells that are contained within human ACI repair tissues several years post-treatment. We h... Read More about Characterization of the cells in repair tissue following autologous chondrocyte implantation in mankind: a novel report of two cases.
Isolation and characterisation of mesenchymal stem cells from different regions of the human umbilical cord. (2013)
Journal Article
Umbilical cords as a source of stem cells are of increasing interest for cell therapies as they present little ethical consideration and are reported to contain immune privileged cells which may be suitable for allogeneic based therapies. Mesenchymal... Read More about Isolation and characterisation of mesenchymal stem cells from different regions of the human umbilical cord..
Isolation and characterization of mesenchymal stem cells from different regions of the human umbilical cord (2013)
Conference Proceeding
The Up-Scale Manufacture of Chondrocytes for Allogeneic Cartilage Therapies
Presentation / Conference
INTRODUCTION: Up-scaled allogeneic cellular products for cartilage therapies, along with identification of novel young donors of healthy chondrocytes, are needed to improve the cost effectiveness and wide-spread use of chondrocyte therapy. METHODS... Read More about The Up-Scale Manufacture of Chondrocytes for Allogeneic Cartilage Therapies.
IS ORTHOPAEDICS LEADING THE WAY IN GETTING CELL THERAPY TO THE CLINIC?
Presentation / Conference
Cell Therapy for Cartilage Repair
Journal Article
Regenerative medicine, using cells as therapeutic agents for the repair or regeneration of tissues and organs, offers great hope for the future of medicine. Cell therapy for treating defects in articular cartilage has been an exemplar of translating... Read More about Cell Therapy for Cartilage Repair.