Divya Chari's Outputs (36)

Fe3O4-PEI-RITC Magnetic Nanoparticles with Imaging and Gene Transfer Capability: Development of a Tool for Neural Cell Transplantation Therapies (2011)
Journal Article
Yiu, H. H. P., Pickard, M. R., Olariu, C. I., Williams, S. R., Chari, D. M., & Rosseinsky, M. J. (2012). Fe3O4-PEI-RITC Magnetic Nanoparticles with Imaging and Gene Transfer Capability: Development of a Tool for Neural Cell Transplantation Therapies. Pharmaceutical Research, 29(5), 1328-1343. https://doi.org/10.1007/s11095-011-0632-1

Purpose
To develop Fe3O4-PEI-RITC magnetic nanoparticles with multimodal MRI-fluorescence imaging and transfection capability, for use in neural cell replacement therapies.

Methods
The Fe3O4-PEI-RITC MNPs were synthesised through a multi-step ch... Read More about Fe3O4-PEI-RITC Magnetic Nanoparticles with Imaging and Gene Transfer Capability: Development of a Tool for Neural Cell Transplantation Therapies.

Magnetic Nanoparticle-Mediated Gene Transfer to Oligodendrocyte Precursor Cell Transplant Populations Is Enhanced by Magnetofection Strategies (2011)
Journal Article
Jenkins, S. I., Pickard, M. R., Granger, N., & Chari, D. M. (2011). Magnetic Nanoparticle-Mediated Gene Transfer to Oligodendrocyte Precursor Cell Transplant Populations Is Enhanced by Magnetofection Strategies. ACS Nano, 5(8), 6527-6538. https://doi.org/10.1021/nn2018717

This study has tested the feasibility of using physical delivery methods, employing static and oscillating field “magnetofection” techniques, to enhance magnetic nanoparticle-mediated gene transfer to rat oligodendrocyte precursor cells derived for t... Read More about Magnetic Nanoparticle-Mediated Gene Transfer to Oligodendrocyte Precursor Cell Transplant Populations Is Enhanced by Magnetofection Strategies.

The transfection of multipotent neural precursor/stem cell transplant populations with magnetic nanoparticles (2010)
Journal Article
Pickard, M. R., Barraud, P., & Chari, D. M. (2011). The transfection of multipotent neural precursor/stem cell transplant populations with magnetic nanoparticles. Biomaterials, 32(9), 2274-2284. https://doi.org/10.1016/j.biomaterials.2010.12.007

Multipotent neural precursor/stem cells (NPCs) are a major transplant population with key properties to promote repair in several neuropathological conditions. Magnetic nanoparticle (MNP)-based vector systems, in turn, offer a combination of key bene... Read More about The transfection of multipotent neural precursor/stem cell transplant populations with magnetic nanoparticles.

Magnetic Nanoparticle Labeling of Astrocytes Derived for Neural Transplantation (2010)
Journal Article
Pickard, M. R., Jenkins, S. I., Koller, C. J., Furness, D. N., & Chari, D. M. (2011). Magnetic Nanoparticle Labeling of Astrocytes Derived for Neural Transplantation. Tissue Engineering Part C: Methods, 17(1), 89-99. https://doi.org/10.1089/ten.tec.2010.0170

Astrocytes are a major transplant cell population to promote neural repair in a range of pathological conditions. In this context, the development of robust methods to label neural transplant populations (for subsequent detection and cell tracking in... Read More about Magnetic Nanoparticle Labeling of Astrocytes Derived for Neural Transplantation.

Enhancement of Magnetic Nanoparticle-Mediated Gene Transfer to Astrocytes by ‘Magnetofection‘: Effects of Static and Oscillating Fields (2010)
Journal Article
Pickard, M., & Chari, D. (2010). Enhancement of Magnetic Nanoparticle-Mediated Gene Transfer to Astrocytes by ‘Magnetofection‘: Effects of Static and Oscillating Fields. Nanomedicine, 5(2), 217-232. https://doi.org/10.2217/nnm.09.109

Aims: To assess the feasibility of using magnetic nanoparticles (MNPs) to transfect astrocytes derived for transplantation and determine if transfection efficacy can be enhanced by static and oscillating magnetic fields. Methods: Astrocytes were tran... Read More about Enhancement of Magnetic Nanoparticle-Mediated Gene Transfer to Astrocytes by ‘Magnetofection‘: Effects of Static and Oscillating Fields.

Uptake of systemically administered magnetic nanoparticles (MNPs) in areas of experimental spinal cord injury (SCI) (2008)
Journal Article
Jeffery, N. D., McBain, S. C., Dobson, J., & Chari, D. M. (2009). Uptake of systemically administered magnetic nanoparticles (MNPs) in areas of experimental spinal cord injury (SCI). Journal of Tissue Engineering and Regenerative Medicine, 3(2), 153-157. https://doi.org/10.1002/term.139

The regenerative potential of the adult central nervous system (CNS) is limited, contributing to poor recovery from neurological insult. Many genes have been identified that promote neural regeneration, but the current viral methods used to mediate n... Read More about Uptake of systemically administered magnetic nanoparticles (MNPs) in areas of experimental spinal cord injury (SCI).

Remyelination In Multiple Sclerosis (2007)
Book Chapter
Chari, D. M. (2007). Remyelination In Multiple Sclerosis. In International Review of Neurobiology (589-620). (79). Elsevier. https://doi.org/10.1016/s0074-7742%2807%2979026-8

Remyelination is the phenomenon by which new myelin sheaths are generated around axons in the adult central nervous system (CNS). This follows the pathological loss of myelin in diseases like multiple sclerosis (MS). Remyelination can restore conduct... Read More about Remyelination In Multiple Sclerosis.

Corticosteroids delay remyelination of experimental demyelination in the rodent central nervous system (2006)
Journal Article
Chari, D. M., Zhao, C., Kotter, M. R., Blakemore, W. F., & Franklin, R. J. (2006). Corticosteroids delay remyelination of experimental demyelination in the rodent central nervous system. Journal of Neuroscience Research, 83(4), 594-605. https://doi.org/10.1002/jnr.20763

High dose corticosteroid (CS) administration is a common mode of therapy in treatment of acute relapses in multiple sclerosis (MS) but the effects of CS on remyelination and the cellular mechanisms mediating this repair process are controversial. We... Read More about Corticosteroids delay remyelination of experimental demyelination in the rodent central nervous system.

Oligodendrocyte progenitor cell (OPC) transplantation is unlikely to offer a means of preventing X-irradiation induced damage in the CNS (2006)
Journal Article
Chari, D. M., Gilson, J. M., Franklin, R. J., & Blakemore, W. F. (2006). Oligodendrocyte progenitor cell (OPC) transplantation is unlikely to offer a means of preventing X-irradiation induced damage in the CNS. Experimental Neurology, 198(1), 145-153. https://doi.org/10.1016/j.expneurol.2005.11.023

Oligodendrocyte lineage cells [oligodendrocytes and their parent cells, the oligodendrocyte progenitor cells (OPCs)] are depleted by X-irradiation and progenitor cell transplantation has been proposed as a therapeutic strategy to counteract radiation... Read More about Oligodendrocyte progenitor cell (OPC) transplantation is unlikely to offer a means of preventing X-irradiation induced damage in the CNS.

Decline in Rate of Colonization of Oligodendrocyte Progenitor Cell (OPC)-Depleted Tissue by Adult OPCs with Age (2003)
Journal Article
Chari, D. M., Crang, A. J., & Blakemore, W. F. (2003). Decline in Rate of Colonization of Oligodendrocyte Progenitor Cell (OPC)-Depleted Tissue by Adult OPCs with Age. Journal of Neuropathology and Experimental Neurology, 62(9), 908-916. https://doi.org/10.1093/jnen/62.9.908

Rates of remyelination decline with age and this has been attributed to slower recruitment of oligodendrocyte progenitor cells (OPCs) into areas of demyelination and slower differentiation of OPCs into remyelinating oligodendrocytes. When considering... Read More about Decline in Rate of Colonization of Oligodendrocyte Progenitor Cell (OPC)-Depleted Tissue by Adult OPCs with Age.

Dysfunctional oligodendrocyte progenitor cell (OPC) populations may inhibit repopulation of OPC depleted tissue (2003)
Journal Article
Chari, D., Huang, W., & Blakemore, W. (2003). Dysfunctional oligodendrocyte progenitor cell (OPC) populations may inhibit repopulation of OPC depleted tissue. Journal of Neuroscience Research, 73(6), 787-793. https://doi.org/10.1002/jnr.10700

We have attempted to extend a previously described rat model of focal oligodendrocyte progenitor cell (OPC) depletion, using 40 Gy X-irradiation (Chari and Blakemore [2002] Glia 37:307–313), to the adult mouse spinal cord, to examine the ability of O... Read More about Dysfunctional oligodendrocyte progenitor cell (OPC) populations may inhibit repopulation of OPC depleted tissue.

New insights into remyelination failure in multiple sclerosis: implications for glial cell transplantation (2002)
Journal Article
Chari, D., & Blakemore, W. (2002). New insights into remyelination failure in multiple sclerosis: implications for glial cell transplantation. Multiple Sclerosis, 8(4), 271-277. https://doi.org/10.1191/1352458502ms842oa

This review considers aspects of remyelination that require further clarification if successful strategies are to be devised to enhance remyelination in multiple sclerosis (MS). We speculate, based on our understanding of the rate with which oligoden... Read More about New insights into remyelination failure in multiple sclerosis: implications for glial cell transplantation.

Modelling large areas of demyelination in the rat reveals the potential and possible limitations of transplanted glial cells for remyelination in the CNS (2002)
Journal Article
Blakemore, W., Chari, D., Gilson, J., & Crang, A. (2002). Modelling large areas of demyelination in the rat reveals the potential and possible limitations of transplanted glial cells for remyelination in the CNS. Glia, 38(2), 155-168. https://doi.org/10.1002/glia.10067

Transplantation of myelin-forming glial cells may provide a means of achieving remyelination in situations in which endogenous remyelination fails. For this type of cell therapy to be successful, cells will have to migrate long distances in normal ti... Read More about Modelling large areas of demyelination in the rat reveals the potential and possible limitations of transplanted glial cells for remyelination in the CNS.

Efficient recolonisation of progenitor‐depleted areas of the CNS by adult oligodendrocyte progenitor cells (2002)
Journal Article
Chari, D. M., & Blakemore, W. F. (2002). Efficient recolonisation of progenitor‐depleted areas of the CNS by adult oligodendrocyte progenitor cells. Glia, 37(4), 307-313. https://doi.org/10.1002/glia.10038

A widely quoted hypothesis for the failure of remyelination in multiple sclerosis (MS) is the exhaustion of the oligodendrocyte progenitor cell (OPC) pool that is strongly implicated as the source of remyelinating oligodendrocytes in demyelinating le... Read More about Efficient recolonisation of progenitor‐depleted areas of the CNS by adult oligodendrocyte progenitor cells.

Depletion of endogenous oligodendrocyte progenitors rather than increased availability of survival factors is a likely explanation for enhanced survival of transplanted oligodendrocyte progenitors in X‐irradiated compared to normal CNS (2001)
Journal Article
Hinks, G. L., Chari, D. M., T. O'Leary, M., Zhao, C., Keirstead, H. S., Blakemore, W. F., & Franklin, R. J. M. (2001). Depletion of endogenous oligodendrocyte progenitors rather than increased availability of survival factors is a likely explanation for enhanced survival of transplanted oligodendrocyte progenitors in X‐irradiated compared to normal CNS. Neuropathology and Applied Neurobiology, 27(1), 59-67. https://doi.org/10.1046/j.0305-1846.2001.00303.x

Oligodendrocyte progenitors (OPs) survive and migrate following transplantation into adult rat central nervous system (CNS) exposed to high levels of X-irradiation but fail to do so if they are transplanted into normal adult rat CNS. In the context o... Read More about Depletion of endogenous oligodendrocyte progenitors rather than increased availability of survival factors is a likely explanation for enhanced survival of transplanted oligodendrocyte progenitors in X‐irradiated compared to normal CNS.

Effect of application of gamma amino butyric acid at the medial preoptic area on sleep-wakefulness (1995)
Journal Article
Chari, D. M., Ramesh, V., John, J., & Mohan Kumar, V. (1995). Effect of application of gamma amino butyric acid at the medial preoptic area on sleep-wakefulness. Indian Journal of Physiology and Pharmacology, 39(3),

Abstract: Intracerebral microinjections of gamma amino butyric acid were Given bilaterally at the medial preoptic area (mPOA) to determine the possible role of this neurotran mitter in the genesis and regulation of leep-wakefulness. GABA (50 Ilg/0.2... Read More about Effect of application of gamma amino butyric acid at the medial preoptic area on sleep-wakefulness.