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Outputs (52)

Transplantation of encapsulated autologous olfactory ensheathing cell populations expressing chondroitinase for spinal cord injury: A safety and feasibility study in companion dogs (2022)
Journal Article

Spinal cord injury (SCI) can cause irreversible paralysis, with no regenerative treatment clinically available. Dogs with natural SCI present an established model and can facilitate translation of experimental findings in rodents to people. We cond... Read More about Transplantation of encapsulated autologous olfactory ensheathing cell populations expressing chondroitinase for spinal cord injury: A safety and feasibility study in companion dogs.

Developing a New Strategy for Delivery of Neural Transplant Populations Using Precursor Cell Sprays and Specialized Cell Media (2021)
Journal Article

Neural precursor/stem cell transplantation therapies promote regeneration in neurological injuries, but current cell delivery methods have drawbacks. These include risks with surgical microinjection (e.g., hemorrhage, embolism) and high cell loss wit... Read More about Developing a New Strategy for Delivery of Neural Transplant Populations Using Precursor Cell Sprays and Specialized Cell Media.

Delivery of chondroitinase by canine mucosal olfactory ensheathing cells alongside rehabilitation enhances recovery after spinal cord injury (2021)
Journal Article

Spinal cord injury (SCI) can cause chronic paralysis and incontinence and remains a major worldwide healthcare burden, with no regenerative treatment clinically available. Intraspinal transplantation of olfactory ensheathing cells (OECs) and injectio... Read More about Delivery of chondroitinase by canine mucosal olfactory ensheathing cells alongside rehabilitation enhances recovery after spinal cord injury.

Enhancing the regenerative potential of stem cell-laden, clinical-grade implants through laminin engineering (2021)
Journal Article

Protected delivery of neural stem cells (NSCs; a major transplant population) within bioscaffolds has the potential to improve regenerative outcomes in sites of spinal cord injury. Emergent research has indicated clinical grade bioscaffolds (e.g. tho... Read More about Enhancing the regenerative potential of stem cell-laden, clinical-grade implants through laminin engineering.

Stiffness-matched biomaterial implants for cell delivery: clinical, intraoperative ultrasound elastography provides a 'target' stiffness for hydrogel synthesis in spinal cord injury (2020)
Journal Article

Safe hydrogel delivery requires stiffness-matching with host tissues to avoid iatrogenic damage and reduce inflammatory reactions. Hydrogel-encapsulated cell delivery is a promising combinatorial approach to spinal cord injury therapy, but a lack of... Read More about Stiffness-matched biomaterial implants for cell delivery: clinical, intraoperative ultrasound elastography provides a 'target' stiffness for hydrogel synthesis in spinal cord injury.

Safe nanoengineering and incorporation of transplant populations in a neurosurgical grade biomaterial, DuraGen PlusTM, for protected cell therapy applications (2020)
Journal Article

High transplant cell loss is a major barrier to translation of stem cell therapy for pathologies of the brain and spinal cord. Encapsulated delivery of stem cells in biomaterials for cell therapy is gaining popularity but experimental research has ov... Read More about Safe nanoengineering and incorporation of transplant populations in a neurosurgical grade biomaterial, DuraGen PlusTM, for protected cell therapy applications.

Electrophysiological properties of neurons grown on soft polymer scaffolds reveal the potential to develop neuromimetic culture environments (2020)
Journal Article

Tissue engineering methodologies for various physiological systems are seeing a significant trend towards 3-D cell culture in or on ‘soft’ polymeric hydrogel materials, widely considered to provide a more biomimetic environment for cell growth versus... Read More about Electrophysiological properties of neurons grown on soft polymer scaffolds reveal the potential to develop neuromimetic culture environments.

Less is more: Investigating the influence of cellular nanoparticle load on transfection outcomes in neural cells (2019)
Journal Article

Genetic engineering of cell transplant populations offers potential for delivery of neurotherapeutic factors to modify the regenerative microenvironment of the injured spinal cord. The use of magnetic nanoparticle (MNP) based vectors has reduced the... Read More about Less is more: Investigating the influence of cellular nanoparticle load on transfection outcomes in neural cells.

Neurosurgical grade biomaterial, DuraGenTM, offers a promising matrix for protected delivery of neural stem cells in clinical cell therapies (2019)
Journal Article

Aims Transplantation of neural stem cells (NSCs) into sites of neurological injury is being investigated in clinical trials around the world. These important self-renewing and multipotent precursors can generate the major central nervous system cell... Read More about Neurosurgical grade biomaterial, DuraGenTM, offers a promising matrix for protected delivery of neural stem cells in clinical cell therapies.

Nanoparticle-Based Imaging of Clinical Transplant Populations Encapsulated in Protective Polymer Matrices (2019)
Journal Article

A recent clinical trial proves that autologous olfactory mucosal cell (OMC) transplantation improves locomotion in dogs with naturally occurring spinal injuries comparable to human lesions. However, not all dogs respond to the treatment, likely due t... Read More about Nanoparticle-Based Imaging of Clinical Transplant Populations Encapsulated in Protective Polymer Matrices.

Noninvasive imaging of nanoparticle-labeled transplant populations within polymer matrices for neural cell therapy (2018)
Journal Article

Aim: To develop a 3D neural cell construct for encapsulated delivery of transplant cells; develop hydrogels seeded with magnetic nanoparticle (MNP)-labeled cells suitable for cell tracking by MRI. Materials & methods: Astrocytes were exogenously labe... Read More about Noninvasive imaging of nanoparticle-labeled transplant populations within polymer matrices for neural cell therapy.

Electrophysiological assessment of primary cortical neurons genetically engineered using iron oxide nanoparticles (2017)
Journal Article

The development of safe technologies to genetically modify neurons is of great interest in regenerative neurology, for both translational and basic science applications. Such approaches have conventionally been heavily reliant on viral transduction m... Read More about Electrophysiological assessment of primary cortical neurons genetically engineered using iron oxide nanoparticles.

A fusion of minicircle DNA and nanoparticle delivery technologies facilitates therapeutic genetic engineering of autologous canine olfactory mucosal cells. (2017)
Journal Article

Olfactory ensheathing cells (OECs) promote axonal regeneration and improve locomotor function when transplanted into the injured spinal cord. A recent clinical trial demonstrated improved motor function in domestic dogs with spinal injury following a... Read More about A fusion of minicircle DNA and nanoparticle delivery technologies facilitates therapeutic genetic engineering of autologous canine olfactory mucosal cells..

Magnetic Nanoparticle-Mediated Gene Delivery to Two- and Three-Dimensional Neural Stem Cell Cultures: Magnet-Assisted Transfection and Multifection Approaches to Enhance Outcomes. (2017)
Book Chapter

Neural stem cells (NSCs) have high translational potential in transplantation therapies for neural repair. Enhancement of their therapeutic capacity by genetic engineering is an important goal for regenerative neurology. Magnetic nanoparticles (MNPs)... Read More about Magnetic Nanoparticle-Mediated Gene Delivery to Two- and Three-Dimensional Neural Stem Cell Cultures: Magnet-Assisted Transfection and Multifection Approaches to Enhance Outcomes..

Part II: Functional Delivery of a Neurotherapeutic Gene to Neural Stem Cells using Minicircle DNA and Nanoparticles: Translational advantages for Regenerative Neurology (2016)
Journal Article

Both neurotrophin-based therapy and neural stem cell (NSC)-based strategies have progressed to clinical trials for treatment of neurological diseases and injuries. Brain-derived neurotrophic factor (BDNF) in particular can confer neuroprotective and... Read More about Part II: Functional Delivery of a Neurotherapeutic Gene to Neural Stem Cells using Minicircle DNA and Nanoparticles: Translational advantages for Regenerative Neurology.

Part I: Minicircle vector technology limits DNA size restrictions on ex vivo gene delivery using nanoparticle vectors: Overcoming a translational barrier in neural stem cell therapy (2016)
Journal Article

Genetically engineered neural stem cell (NSC) transplant populations offer key benefits in regenerative neurology, for release of therapeutic biomolecules in ex vivo gene therapy. NSCs are ‘hard-to-transfect’ but amenable to ‘magnetofection’. Despite... Read More about Part I: Minicircle vector technology limits DNA size restrictions on ex vivo gene delivery using nanoparticle vectors: Overcoming a translational barrier in neural stem cell therapy.

Using a 3-D multicellular simulation of spinal cord injury with live cell imaging to study the neural immune barrier to nanoparticle uptake (2016)
Journal Article

Development of nanoparticle (NP) based therapies to promote regeneration in sites of central nervous system (CNS; i.e. brain and spinal cord) pathology relies critically on the availability of experimental models that offer biologically valid predict... Read More about Using a 3-D multicellular simulation of spinal cord injury with live cell imaging to study the neural immune barrier to nanoparticle uptake.

Development and characterisation of neurocompatible transfection grade magnetic nanoparticles for genetic modification of neural stem cells (2016)
Journal Article

Genetic modification of cell transplant populations and cell tracking ability are key underpinnings for effective cell therapies. Current strategies to achieve these goals utilize methods which are unsuitable for clinical translation because of relat... Read More about Development and characterisation of neurocompatible transfection grade magnetic nanoparticles for genetic modification of neural stem cells.

Development of Multifunctional Magnetic Nanoparticles for Genetic Engineering and Tracking of Neural Stem Cells (2016)
Journal Article

Genetic modification of cell transplant populations and cell tracking ability are key underpinnings for effective cell therapies. Current strategies to achieve these goals utilize methods which are unsuitable for clinical translation because of relat... Read More about Development of Multifunctional Magnetic Nanoparticles for Genetic Engineering and Tracking of Neural Stem Cells.

Endocytotic potential governs magnetic particle loading in dividing neural cells: studying modes of particle inheritance. (2016)
Journal Article

AIM: To achieve high and sustained magnetic particle loading in a proliferative and endocytotically active neural transplant population (astrocytes) through tailored magnetite content in polymeric iron oxide particles. MATERIALS & METHODS: MPs of var... Read More about Endocytotic potential governs magnetic particle loading in dividing neural cells: studying modes of particle inheritance..

'Stealth' nanoparticles evade neural immune cells but also evade all major brain cell populations: Implications for PEG-based neurotherapeutics (2016)
Journal Article

Surface engineering to control cell behavior is of high interest across the chemical engineering, drug delivery and biomaterial communities. Defined chemical strategies are necessary to tailor nanoscale protein interactions/adsorption, enabling contr... Read More about 'Stealth' nanoparticles evade neural immune cells but also evade all major brain cell populations: Implications for PEG-based neurotherapeutics.

Increasing magnetite contents of polymeric magnetic particles dramatically improves labeling of neural stem cell transplant populations. (2015)
Journal Article

Safe and efficient delivery of therapeutic cells to sites of injury/disease in the central nervous system is a key goal for the translation of clinical cell transplantation therapies. Recently, 'magnetic cell localization strategies' have emerged as... Read More about Increasing magnetite contents of polymeric magnetic particles dramatically improves labeling of neural stem cell transplant populations..

Deploying clinical grade magnetic nanoparticles with magnetic fields to magnetolabel neural stem cells in adherent versus suspension cultures (2015)
Journal Article

Neural stem cells (NSCs) have a high therapeutic potential for patients with neurological disease/injury given their neuroregenerative and immunomodulatory capabilities. In recent years, magnetic nanoparticles (MNPs) have been used as contrast agents... Read More about Deploying clinical grade magnetic nanoparticles with magnetic fields to magnetolabel neural stem cells in adherent versus suspension cultures.

Using magnetic nanoparticles for gene transfer to neural stem cells: stem cell propagation method influences outcomes (2015)
Journal Article

Genetically engineered neural stem cell (NSC) transplants offer a key strategy to augment neural repair by releasing therapeutic biomolecules into injury sites. Genetic modification of NSCs is heavily reliant on viral vectors but cytotoxic effects ha... Read More about Using magnetic nanoparticles for gene transfer to neural stem cells: stem cell propagation method influences outcomes.

Magnetic nanoparticles for oligodendrocyte precursor cell transplantation therapies: progress and challenges (2014)
Journal Article

Oligodendrocyte precursor cells (OPCs) have shown high promise as a transplant population to promote regeneration in the central nervous system, specifically, for the production of myelin - the protective sheath around nerve fibers. While clinical tr... Read More about Magnetic nanoparticles for oligodendrocyte precursor cell transplantation therapies: progress and challenges.

Identifying the cellular targets of drug action in the central nervous system following corticosteroid therapy (2014)
Journal Article

Corticosteroid (CS) therapy is used widely in the treatment of a range of pathologies, but can delay production of myelin, the insulating sheath around central nervous system nerve fibers. The cellular targets of CS action are not fully understood, t... Read More about Identifying the cellular targets of drug action in the central nervous system following corticosteroid therapy.

Magnetic nanoparticle mediated transfection of neural stem cell suspension cultures is enhanced by applied oscillating magnetic fields. (2013)
Journal Article

UNLABELLED: Safe genetic modification of neural stem cell (NSC) transplant populations is a key goal for regenerative neurology. We describe a technically simple and safe method to increase transfection in NSCs propagated in the neurosphere (suspensi... Read More about Magnetic nanoparticle mediated transfection of neural stem cell suspension cultures is enhanced by applied oscillating magnetic fields..

Alignment of multiple glial cell populations in 3D nanofiber scaffolds: Toward the development of multicellular implantable scaffolds for repair of neural injury (2013)
Journal Article

Non-neuronal cells of the central nervous system (CNS), termed “neuroglia,” play critical roles in neural regeneration; therefore, replacement of glial populations via implantable nanofabricated devices (providing a growth-permissive niche) is a prom... Read More about Alignment of multiple glial cell populations in 3D nanofiber scaffolds: Toward the development of multicellular implantable scaffolds for repair of neural injury.

Differences in magnetic particle uptake by CNS neuroglial subclasses: implications for neural tissue engineering (2013)
Journal Article

AIM: To analyze magnetic particle uptake and intracellular processing by the four main non-neuronal subclasses of the CNS: oligodendrocyte precursor cells; oligodendrocytes; astrocytes; and microglia. MATERIALS & METHODS: Magnetic particle uptake and... Read More about Differences in magnetic particle uptake by CNS neuroglial subclasses: implications for neural tissue engineering.