The Human Mesenchymal Stem Cell Secretome Directs Macrophage Differentiation in an Oxygen-Dependent Manner (2023)
Preprint / Working Paper
Shephard, M. T., Merkhan, M. M., Dale, T. P., & Forsyth, N. R. (2023). The Human Mesenchymal Stem Cell Secretome Directs Macrophage Differentiation in an Oxygen-Dependent Manner

Background
Chronic disease states can share inflammation as an underlying pathology. Macrophages are associated with chronic inflammation; in general, M1 phenotype macrophage activity inhibits cell proliferation and causes tissue damage whilst M2 ma... Read More about The Human Mesenchymal Stem Cell Secretome Directs Macrophage Differentiation in an Oxygen-Dependent Manner.

Heparin inhibits cellular invasion by SARS-CoV-2: structural dependence of the interaction of the surface protein (spike) S1 receptor binding domain with heparin (2020)
Preprint / Working Paper
Mycroft-West, C. J., Su, D., Pagani, I., Rudd, T. R., Elli, S., Guimond, S. E., Miller, G., Meneghetti, M. C. Z., Nader, H. B., Li, Y., Nunes, Q. M., Procter, P., Mancini, N., Clementi, M., Bisio, A., Forsyth, N. R., Turnbull, J. E., Guerrini, M., Fernig, D. G., Vicenzi, E., …Skidmore, M. A. Heparin inhibits cellular invasion by SARS-CoV-2: structural dependence of the interaction of the surface protein (spike) S1 receptor binding domain with heparin

Glycosaminoglycans induce conformational change in the SARS-CoV-2 Spike S1 Receptor Binding Domain (2020)
Journal Article
Mycroft-West, C. J., Su, D., Li, Y., Guimond, S. E., Rudd, T. R., Elli, S., Miller, G., Nunes, Q. M., Procter, P., Bisio, A., Forsyth, N. R., Turnbull, J. E., Guerrini, M., Fernig, D. G., Yates, E. A., Lima, M. A., & Skidmore, M. A. Glycosaminoglycans induce conformational change in the SARS-CoV-2 Spike S1 Receptor Binding Domain. bioRxiv, https://doi.org/10.1101/2020.04.29.068767

The glycosaminoglycan (GAG) class of polysaccharides are utilised by a plethora of microbial pathogens as receptors for adherence and invasion. The GAG heparin prevents infection by a range of viruses when added exogenously, including the S-associate... Read More about Glycosaminoglycans induce conformational change in the SARS-CoV-2 Spike S1 Receptor Binding Domain.

SARS-CoV-2 Spike S1 Receptor Binding Domain undergoes Conformational Change upon Interaction with Low Molecular Weight Heparins (2020)
Journal Article
Mycroft-West, C. J., Su, D., Li, Y., Guimond, S. E., Rudd, T. R., Elli, S., Miller, G., Nunes, Q. M., Procter, P., Bisio, A., Forsyth, N. R., Turnbull, J. E., Guerrini, M., Fernig, D. G., Yates, E. A., Lima, M. A., & Skidmore, M. A. SARS-CoV-2 Spike S1 Receptor Binding Domain undergoes Conformational Change upon Interaction with Low Molecular Weight Heparins. arXiv, https://doi.org/10.1101/2020.04.29.068486

The dependence of the host on the interaction of hundreds of extracellular proteins with the cell surface glycosaminoglycan heparan sulphate (HS) for the regulation of homeostasis is exploited by many microbial pathogens as a means of adherence and i... Read More about SARS-CoV-2 Spike S1 Receptor Binding Domain undergoes Conformational Change upon Interaction with Low Molecular Weight Heparins.