Watkinson
Photoelectrochemical Imaging System for the Mapping of Cell Surface Charges
Watkinson
Authors
Abstract
The surface charge of cells affects cell signaling, cell metabolic processes, adherence to surfaces, and cell proliferation. Our understanding of the role of membrane charges is limited due to the inability to observe changes without interfering, chemically or physically, with the cell or its membrane. Here, we report that a photoelectrochemical imaging system (PEIS) based on label-free ac-photocurrent measurements at indium tin oxide (ITO) coated glass substrates can be used to map the basal surface charge of single live cells under physiological conditions. Cells were cultured on the ITO substrate. Photocurrent images were generated by scanning a focused, modulated laser beam across the back of the ITO coated glass substrate under an applied bias voltage. The photocurrent was shown to be sensitive to the negative surface charge of the substrate facing, basal side of a single living cell-an area not accessible to other electrochemical or electrophysiological imaging techniques. The PEIS was used to monitor the lysis of mesenchymal stem cells.
Citation
Watkinson. (2019). Photoelectrochemical Imaging System for the Mapping of Cell Surface Charges. Analytical Chemistry, 5896 -5903. https://doi.org/10.1021/acs.analchem.9b00304
Acceptance Date | Apr 15, 2019 |
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Publication Date | May 7, 2019 |
Journal | Analytical Chemistry |
Print ISSN | 0003-2700 |
Publisher | American Chemical Society |
Pages | 5896 -5903 |
DOI | https://doi.org/10.1021/acs.analchem.9b00304 |
Publisher URL | http://doi.org/10.1021/acs.analchem.9b00304 |
Files
single cell imaging with ITO -accepted.docx
(11.2 Mb)
Document
Publisher Licence URL
https://creativecommons.org/licenses/by-nc/4.0/
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