Regulating the expression of gene drives is key to increasing their invasive potential and the mitigation of resistance (2021)
Journal Article
Hammond, A., Karlsson, X., Morianou, I., Kyrou, K., Beaghton, A., Gribble, M., …Nolan, T. (2021). Regulating the expression of gene drives is key to increasing their invasive potential and the mitigation of resistance. PLoS Genetics, e1009321 - ?. https://doi.org/10.1371/journal.pgen.1009321

Homing-based gene drives use a germline source of nuclease to copy themselves at specific target sites in a genome and bias their inheritance. Such gene drives can be designed to spread and deliberately suppress populations of malaria mosquitoes by i... Read More about Regulating the expression of gene drives is key to increasing their invasive potential and the mitigation of resistance.

The creation and selection of mutations resistant to a gene drive over multiple generations in the malaria mosquito (2017)
Journal Article
M. Hammond, A., Kyrou, K., Bruttini, M., North, A., Galizi, R., Karlsson, X., …Nolan, T. (2017). The creation and selection of mutations resistant to a gene drive over multiple generations in the malaria mosquito. PLoS Genetics, e1007039 - ?. https://doi.org/10.1371/journal.pgen.1007039

Gene drives have enormous potential for the control of insect populations of medical and agricultural relevance. By preferentially biasing their own inheritance, gene drives can rapidly introduce genetic traits even if these confer a negative fitness... Read More about The creation and selection of mutations resistant to a gene drive over multiple generations in the malaria mosquito.

A CRISPR-Cas9 sex-ratio distortion system for genetic control (2016)
Journal Article
Galizi, R., Hammond, A., Kyrou, K., Taxiarchi, C., Bernardini, F., O’Loughlin, S. M., …Crisanti, A. (2016). A CRISPR-Cas9 sex-ratio distortion system for genetic control. Scientific Reports, 31139 - ?. https://doi.org/10.1038/srep31139

Genetic control aims to reduce the ability of insect pest populations to cause harm via the release of modified insects. One strategy is to bias the reproductive sex ratio towards males so that a population decreases in size or is eliminated altogeth... Read More about A CRISPR-Cas9 sex-ratio distortion system for genetic control.

The germline of the malaria mosquito produces abundant miRNAs, endo-siRNAs, piRNAs and 29-nt small RNAs. (2015)
Journal Article
Castellano, L., Rizzi, E., Krell, J., Di Cristina, M., Galizi, R., Mori, A., …Nolan, T. (2015). The germline of the malaria mosquito produces abundant miRNAs, endo-siRNAs, piRNAs and 29-nt small RNAs. BMC Genomics, 100 - ?. https://doi.org/10.1186/s12864-015-1257-2

BACKGROUND: Small RNAs include different classes essential for endogenous gene regulation and cellular defence against genomic parasites. However, a comprehensive analysis of the small RNA pathways in the germline of the mosquito Anopheles gambiae ha... Read More about The germline of the malaria mosquito produces abundant miRNAs, endo-siRNAs, piRNAs and 29-nt small RNAs..

Site-specific genetic engineering of the Anopheles gambiae Y chromosome. (2014)
Journal Article
Bernardini, F., Galizi, R., Menichelli, M., Papathanos, P., Dritsou, V., Marois, E., …Windbichler, N. (2014). Site-specific genetic engineering of the Anopheles gambiae Y chromosome. Proceedings of the National Academy of Sciences of the United States of America, 7600 - 7605. https://doi.org/10.1073/pnas.1404996111

Despite its function in sex determination and its role in driving genome evolution, the Y chromosome remains poorly understood in most species. Y chromosomes are gene-poor, repeat-rich and largely heterochromatic and therefore represent a difficult t... Read More about Site-specific genetic engineering of the Anopheles gambiae Y chromosome..